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Electroporation

Electroporation Homestead

FUN FACT: The first demonstration that electroporation could be used for transfection and expression of a foreign gene was made in 1982, and the term electroporation was coined.

What is Electroporation?

Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, electrode arrays or DNA to be introduced into the cell.

What is the use of Electroporation?

Initially developed for gene transfer, electroporation is now in use for delivery of a large variety of molecules: From ions to drugs, dyes, tracers, antibodies, and oligonucleotides to RNA and DNA.

What are the advantages and disadvantages of electroporation?

Electroporation has several advantages: versatility (works with any cell type), efficiency, very low DNA requirements, and the ability to operate in living organisms. Disadvantages include potential cell damage and the nonspecific transport of molecules into and out of the cell.

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